|
KMID : 0379520000160020125
|
|
한국독성학회지
2000 Volume.16 No. 2 p.125 ~ p.131
|
|
|
Effects of Rhus verniciflua Stokes (RVS) on Cell-associated Detoxificant Enzymes and Glucose Oxidase-mediated Toxicity in Cultured Mouse Hepatocytes
|
|
Lim Kye-Taek
Lee Jeong-Chae
Jung Hee-Young
Jo Sung-Kyun
|
|
|
Abstract
|
|
|
|
The ethanol extract of Rhus verniciflua Stokes (RVS), the Korean Lacquer tree, was subsequentely isolated and fractioned into two portions using distilled water (SED) and 99% ethanol (SEE) as elution buffers through silica gel column (4x28 em, 22 AA. 28~200 mesh). To know the antioxidative effect of the RVS extracts, primary hepatocytes were exposed to hydroxyl radical generated by 20 mU/mell glucose oxidase with SED or SEE for 4 hr. The addition of 100mutextrm{g/mell SED in culture medium showed good protection from glucose oxidase (GO)-mediated cytotoxicity of hepatocytes, showing approximately equivalent to control. When the hepatocytes were incubated with 100 mutextrm{g}/mell SED or SEE only for 4 hr. the activities of cell-associated superoxide dismutase (SOD) and catalase were elevated up to 1.22 fold and 1.4 fold, respectively, compared to control. Further increase, 1.88fold in SOD activity or 1.64fold in catalase activity, was also observed when the hepatocytes were incubated with 100 units/mell of commercial SOD or catalase for 4 hr. Moreover. the glucose oxidase-mediated cytotoxicity in cultured hepatocytes was generally reduced upon addition of lysate obtained from SED or SEE-stimulated hepatocytes in a dose-dependent manner. From these results, we suggest that, in cultured hepatocytes, RVS ethanol extract can efficiently reduce cytotoxicity induced by glucose oxidase and may increase the activity of cell-associated SOD and/or catalase, thereby preventing and/or scavenging superoxides and hydroxyl radicals in this experiment.
|
|
|
KEYWORD
|
|
|
Rhus verniciflua Stokes (RVS) ethanol extract, Hepatocytes, Glucose oxidase, Catalase, Superoxide dismutase
|
|
|
FullTexts / Linksout information
|
|
|
|
|
Listed journal information
|
|
 
|
|